Bipolaris Stem Rot of Cactus Caused by Bipolaris cactivora (Petrak) Alcorn  [1998]

Chang MeeHyun Ik-Hwa Overseas Pest Division, National Institute of Agricultural Science and Technology, RDA, Suwon (Korea Republic)

Bipolaris stem rot of cactus severely occurred up to 77% at the field of Koyang and Kimcheon from 1996 to 1997. The symptom was initially light yellow, water soaked round lesion, subsequently turned light brown and dried to death. The causal fungus was identified as Bipolaris cactivora (Petrak) Alcorn. Conidia we


re obclavare to fusoid, rounded ends, light brown color, 1~4 septate, and conidial size was 23~42*6~9 micro m (av. 32.5*7.5 micro m). Conidiophores were inoculated with the isolates obtained from the lesion of diseased plants, the same characteristic symptoms as those in the field were produced. The symptom of four-month-old cactus was developed more rapidly than that of six-month-old cactus. The pathogen was reisolated from the artificially inoculated lesions.

A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7�� and 95.1��, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.


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